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Paul Ryan’s budget proposal, which would convert Medicare into a sort of voucher system. Sen. Pat Toomey also proposed his personal budget with even deeper health cuts . Politico Pro: GOP Eyes Mandate As A Deficit Decrease Device Supporters of President Barack Obama’ health care law watch its insurance requirement as the most effective method to expand coverage of health. Republicans, however, see it as something entirely different: a possible source of savings in the forthcoming super committee deficit talks. Republicans have generally seen the law’ individual mandate as a political weapon -; a symbol of big-government overreach that they can make use of to turn up the GOP base and keep raising doubts about regulations among independent voters.Western Blotting Individual glomerular extract or recombinant PLA2R expressed by individual cells was electrophoresed under nonreducing conditions and used in nitrocellulose membranes, according to standard protocols. Human being serum was used as the main antibody, at a dilution of 1 1:100, unless indicated otherwise. A guinea pig polyclonal antibody against the M-type phospholipase A2 receptor grew up against full-duration purified rabbit PLA2R.12 Sheep antibodies against the four IgG subclasses were used as recommended by the product manufacturer . Detecting antibodies had been peroxidase-conjugated donkey antibodies against human being IgG, guinea pig IgG , or sheep IgG .